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The mixed Rex domain NAPstar4.3 for example has no detectable binding affinity for NADH and has a K dNADPH 43 M and a K r(NADPH/NADP+) 0.28, which is approximately one order of magnitude lower than other NAPstar sensors ( Figure 1 ) and allows fully dynamic measurement of NADP oxidation and reduction in the yeast cytosol during the yeast metabolic cycle and in the plant cytosol during illumination and hypoxia ( Figure 3 and 4 )